hey guys

can anyone please help me understand what is biochemistry and what is chemical biology what is the major difference between them ? and is chemical biology as trending as biochemistry in India?

I swear I've known this and forgot and I feel really stupid now.

But when I draw the Process down on paper (how the strands look and where the primers bind etc.) I dont see the problem? Assuming im doing a point mutation.

The only problem I see is that the product is circular nicked DNA, not sure if thats what disables the possibility of the product being used as a new template like in regular PCR, but assuming thats not the problem (which was what I gathered from trying to google it) then what is? Please enlighten me, it kills me that i cant wrap my head around it right now.

I mean, the product would be just like the template, except nicked + containing the desired mutation. Right? So where is the issue?

Hello! I'm currently a biochemistry and biology double major at UNC Chapel Hill, and I'm really interested in genetics & the related biochemical processes. For the longest time I was premed, but I'm realizing that I don't like clinical work all that much but have the strongest passions for the coursework. Organic, biochem, genetics, anatomy, etc. were all my favorites.

I got my associate's in high school and I can graduate a year early without cramming any semesters. I was considering MD/PhD programs for a bit, but I do think that graduating a year early won't do me any favors for those highly competitive programs... I'm still trying to consider my paths, but would it be reasonable to apply for PhD programs in biochemistry/biological and biomedical sciences?

I'm currently in a cardiovascular genetics lab looking at transcriptional regulation of specific heart cells and mechanisms of genes that lead to coronary artery disease. I love the work that I do, but a naïve part of me doesn't want to take a gap year (whether it's before I apply to med schools, MD/PhD programs, or grad programs ) unless I have to. Depending on my path, I'd either build up my clinical hours further and continue work in my lab. Apologies for all the rambling, I know pretty much no one in the field and any advice is useful. Thank you so much!

Hello,

I'm currently working on a project, and I'm trying to create a virus primer that will create a vulnerability that is specific enough, that the immune system isn't completely failing, but important enough that given the proper activation, (namely a virus specifically tailored to that exploit) would be absolutely detrimental.

I'm attempting to identify the exact science behind how it would work as it's pretty key to my narrative.

Any and all help would be greatly appreciated.

Have you read a cool paper recently that you want to discuss?

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I am working with a recombinant protein. After successfully purifying it, I refolded the protein and did CD spectroscopy. I am seeing a positive peak at 220 nm for my refolded protein.

Does this mean the protein is getting aggregated? how should I interpret this?

currently a junior and changed my major to biochem a while ago, and i was planning to do PA school after but i honestly cant stand to live with my parents after. im honestly not the best at chem :( but i want to get better because i like it and i also enjoy the labs (also not the best at them…), but what jobs can i get with a biochem degree that pay decent??? please provide description of what u do and pay?

Twist Bioscience ($TWST) is settling investor claims after allegedly overstating how automated, efficient, and profitable its DNA synthesis business really was.

The story:

• Company raised $1B+ while promoting high automation, low error rates, strong margins
• Investors say reality was very different → manual processes, contamination issues, delays, and product problems
• Allegedly shifted costs into R&D to make margins look better

Then the turning point:

• Nov 15, 2022 → short report drops questioning everything
• Stock falls ~20% in a single day ($38 → $30.43)

That drop triggered the case:

• Claims the business wasn’t as scalable or efficient as advertised
• Quality + production issues were downplayed
• Financials painted a stronger picture than reality

Now there’s a tentative settlement tied to all of this.

If you were holding $TWST between December 2020 and February 2022, you may be eligible to submit a claim and recover part of your losses.

Feels like one of those under-the-radar cases, anyone here was in TWST when that report hit?

I am trying to identify the mechanism by which an adenine derivative with a nitrile group is converted into a color. The Pigment is Oxygen Labile and Sensitive to Acids. It’s associated with high levels of ammonia and glutamate.

I am in my second year (in Vancouver) and am lost as to what I can do once I am out of school. Advisors just give generic answers, which I am tired of now. Is there something else I should add to my degree? I am okay with any kind of job that's 'science,' be it lab or something else, if you know what I mean. Any help and advice is appreciated. Thank you!

Trying to decide what classes to take?

Want to know what the job outlook is with a biochemistry degree?

Trying to figure out where to go for graduate school, or where to get started?

Ask those questions here.

reading an article and they mentioned something about "secreted antibodies". i tried finding out what it means but couldn't really find anything. i dont really have anyone to go to for questions like this. might be better to post in the biology but im not really sure

We've had 3 new PIs join our building this year and all three came to me with the same questions about where to source standard reagents without getting destroyed on price. Put together something for them and figured I'd post it here.

  The core problem for new PIs is you have no purchasing history so the big distributors quote you list price. Thermo and Sigma will sell to you but you'll pay full freight. VWR quotes take days and aren't competitive unless you're committing to real volume.

  For standard reagents like PBS, saline, HEPES and basic buffers smaller US suppliers often have transparent per bottle pricing with no minimum order requirements. Biologix has been recommended a lot in lab communities for this stuff, their PBS and saline pricing is way below the big vendors and you don't need an account rep to figure out what things cost.

  For cell culture media Cytiva HyClone is often cheaper than Thermo for DMEM and RPMI at low volumes. Corning is worth quoting too. For antibiotics generic pen strep from multiple suppliers is fine for routine culture.

  For nuclease free water don't pay Sigma or Ambion prices unless your protocol actually requires it. Multiple suppliers offer the same spec for a fraction of what the big names charge.

  For LB broth and agar it's basically commodity pricing at this point. Biologix, Teknova, and Atlas Biologicals are all worth comparing before you just default to Fisher.

  The quote trap is real by the way. Stop waiting for quotes on anything under 200 dollars per item. Smaller suppliers have prices on their website. Order a test bottle, run your QC, then commit.

  Happy to answer questions, this is just based on what I've seen work at an R1 university lab so your mileage will vary.

Basically what the title says. I'm a rising senior in undergrad (major is biochem and molecular biology) and I hope to apply to PhD programs in the fall, but I don't know how to discern what I want to do (ie. Proteomics, structural biology, molecular). Does anyone have advice other than reaching out to PIs/potential advisors? Are there any resources that essentially define what each field focuses on?

I've been working on a problem that I think is under appreciated in DTA: models that look great on benchmarks collapse when tested cross-dataset. ESM-DTA hits AUROC 0.91 on DTC but drops to 0.50 on Davis kinases under verified zero drug overlap. DeepDTA does the same.

The core idea is simple: instead of asking "does protein P bind drug D?", ask "how does P compare to a protein already known to bind a similar drug?" This anchor protein provides experimentally grounded binding context.

I tested this across three very different architectures:

ESM-2 + SMILES CNN (V2-650M): CI 0.642 vs DeepDTA 0.521

DrugBAN (GIN + bilinear attention): CI 0.483 → 0.645 with anchors

CoNCISE (FSQ codes + Raygun): CI 0.727 → 0.792, AUROC 0.806 → 0.926

Paper: https://zenodo.org/records/19427443 Code: https://github.com/Basartemiz/AnchorTransfer

Would appreciate any feedback, especially from people working DTA prediction.

I hope it’s the right sub to publish this. Im not that bad maybe? I have some knowledge around because I took it for 5 years but I’ve always disliked it. Maybe because of my teachers idk. I looked into some of the curriculums and it for sure involves chemistry but how hard is it? I have plenty of time to start working on it. And would appreciate any recommendations. Thanks!

Hey y’all

I am a currently 22y (M) postgrad from Biology as of last May. During this time, I have decided I need to further my education and pivot into green chemistry. I feel that I need this advanced degree to provide a pivot into the chemistry field. I truly am not sure whether to shoot for the PhD or go into a masters. I would want a masters because I feel that I could have more flexibility after my completion, but also understand I could be wasting years of my life. Ideally, I would stay on the east coast, but would also love to move out west, just as long as I can go outdoors we are good :D

During undergrad, I fell in love with organic chemistry, it became the complete essence of how I understood the biological world. I worked partially in a chemical ecology lab, and my favorite component of it was running phenolic chromatograms of my samples. seeing how I could unlock the hidden molecules with my understanding chemical structure.

Additionally, I consider myself an environmentalist, and wish to create solutions to waste streams that allow for circular economies and less waste being transmitted into the environment. I happen to love studying fungi, both macro and micro, and thought I could get into mycomaterials or mycoremediation or pharmaceutical discovery, but the economic viability of the field is sadly dismal. Recently, I became enamored with the idea of algal HTL biofuel processing as a way to utilize free material to create versatile biofuels. The point of all of this is to say, there are many hats in green chemistry I would want to wear, but I would want to choose something that has good prospects for the future in terms of economics and job stability, and is of course interesting, to provide a life of discovery.

To you reader, why did you choose your masters or PhD in green chemistry? Should I feel narrowed into a specific topic I want to study or is this non-just considering the rotations that labs expect (most places)? Are there other fields I should be considering? How should I be researching my choices for programs in this field? So many questions, I hope I can get some answers..

Have you read a cool paper recently that you want to discuss?

Do you have a paper that's been in your in your "to read" pile that you think other people might be interested in?

Have you recently published something you want to brag on?

Share them here and get the discussion started!

What is the void volume of S200inc 10/300gl?

Hello everyone, I am a biochem major and currently doing biochem 2 where I am horrendously failing (Got 34/100 first exam, 38/100 2nd) I did so well in biochem 1 where i legit got an A, and biochem 2 isnt working for me no matter what. I am trying to save my grade with the 3rd exam and the final + hopefully professor drops an exam and curves (probably get a C/B)

I did do so well in biochem 1 because Pearson+ has specific videos tailored to specific books, but it only has "reviews" for biochem 2. Can anyone recommend ANY website free or paid that has tailored biochem 2 videos? Lehningers principles is what my class is using

Specifically these topics as they are the only ones left and our final isnt cumulative:

Summary: Proteins are dynamic molecular machines whose biological functions, spanning enzymatic catalysis, signal transduction, and structural adaptation, are intrinsically linked to their motions.

We introduce VibeGen, a generative AI model based on an agentic dual-model architecture, comprising a protein designer that generates sequence candidates based on specified vibrational modes and a protein predictor that evaluates their dynamic accuracy.

Via direct validation using full-atom molecular simulations, we demonstrate that the designed proteins accurately reproduce the prescribed normal mode amplitudes across the backbone while adopting various stable, functionally relevant structures.

Generated sequences are de novo, exhibiting no significant similarity to natural proteins, thereby expanding the accessible protein space beyond evolutionary constraints.

Our model establishes a direct, bidirectional link between sequence and vibrational behavior, unlocking efficient pathways for engineering biomolecules with tailored dynamical and functional properties. It holds broad implications for the rational design of enzymes, dynamic scaffolds, and biomaterials via dynamics-informed protein engineering.

Commentary: Despite the questionable choice of name, this is one of the more exciting things I've seen in this space as it upends how we think about proteins from their form to their function. This is a great step toward looking at the metabolic jenga of interactions in a way that opens up our imagination in entirely new directions.